Development and validation of bexarote by bioanalytical methods using liquid chromatography-tandem mass spectroscopy (LC-MS/MS)

Abstract Background The aim of this study was to develop and validate accurate precise UPLC method with tandem mass spectrometry (Waters) for the determination bexarotene in human plasma using D4 as internal standard (IS). Results retention time 2.75 ± 0.30 min. validated respect system suitability, linearity, accuracy, precision, matrix effect, auto sampler carryover test, recovery. Linearity found be 1.04 351.93 μg/mL. LOQQC, LQC, INTQC, MQC, HQC were 1.0550, 2.7800, 25.2700, 131.61, 263.23 respectively. mean percentage recovery 95.72% Conclusion bioanalytical method, a selective sensitive liquid chromatography-mass quantitate K 2 EDTA over concentration range 1.0440 351.9320 ng/mL, successfully validated. This is suitable sample analysis support bioequivalence/bioavailability and/or pharmacokinetic studies involving formulations bexarotene.